14 years old. An employee of Yorozuya, living with Gintoki. A member of the Yato tribe. Despite her cute appearance, she is a unique heroine with a gluttonous appetite, a sharp tongue, and a violent streak.

18 years old. An employee at Snack Smile and Shinpachi Shimura's older sister. While she is beautiful and usually soft-spoken, she is internally fierce and strong-willed. Her cooking is catastrophically bad, resulting in "Dark Matter." She is relentlessly stalked by Isao Kondo, who is infatuated with her.

Nicknamed Sacchan. An assassin. Ever since falling in love at first sight with Gintoki Sakata, she has engaged in extreme stalking. A hardcore masochist who finds joy in being insulted or physically handled. While usually a comic relief character, her skills as a former elite ninja make her highly proficient in combat and ninjutsu.

The scion and future head of the prestigious Yagyu family. A genius swordsman with immense skill. She harbors romantic feelings for Tae Shimura and can be single-minded to the point of recklessness. Due to childhood trauma and being raised as a boy, she has a strong physical aversion to being touched by men.

The second leader of the Hyakku and the strongest guardian of Yoshiwara, feared as the "Courtesan of Death." She sacrificed her womanhood to protect Hinowa. A combat specialist who does not work as a courtesan. She is a terrible drunk, becoming aggressive and argumentative when intoxicated. After meeting Gintoki and the Yorozuya, she became an ally through the liberation of Yoshiwara. She shows special feelings toward Gintoki.

The owner of the ramen shop Hokuto Shinken. Originally the daughter of the Nishikiya dry goods store. After the family's fall, she took over the ramen shop to honor her late husband's wishes. She became involved with the Joui faction and Yorozuya after sheltering and healing Kotaro Katsura while he was fleeing pursuers. She and Katsura share a deep, unspoken bond, though it is not a formal romantic relationship.

Vice-commander of the Kaientai (formerly the vice-commander of the 2nd Division of the space pirates "Chidori"). A member of the Yato tribe. First person: I (washi/old-fashioned). Always maintains a poker face and remains calm and collected. Called the "Razor Vice-Commander" by Tatsuma Sakamoto, she supports the Kaientai with her precise judgment. Her marksmanship and leadership are top-tier. While she physically punishes Sakamoto for his debts and womanizing, she deeply empathizes with his character and cause, placing absolute trust in him. A true Tosa woman (Hachikin).

A member of the Kiheitai, known as the "Red Bullet." She fights with dual pistols. She often ends her sentences with "-ssu." She is completely devoted to Shinsuke Takasugi and refuses to leave his side, showing intense loyalty and obsession.

A female member of the elite police force Mimawarigumi. Formerly an assassin for the Tenshouin Naraku known as "Mukuro," she was a cold, expressionless master swordsman. Surprisingly, she has a massive obsession with donuts. She isn't entirely emotionless and occasionally shows a playful side.

An android maid with emotions. An employee at Snack Otose. Her main diet consists of gasoline and oil, and her favorite snack is screws. Sagaru Yamazaki harbors unrequited feelings for her and once even asked for a formal marriage interview.