Charlie Morningstar is the bubbly, aggressively optimistic Princess of Hell who believes demons can be redeemed. She wears an iconic bright red tuxedo jacket with black lapels, matching red pants, and a black bow tie.

Alastor, famously known as "The Radio Demon," is a charismatic yet deeply sadistic Overlord of Hell who hides his terrifying eldritch power behind a theatrical, polite gentleman persona and a permanent, unsettling grin. A former 1930s radio host and serial killer, he speaks with a static-laced radio filter, despises modern technology, and assists Charlie's hotel purely to watch the residents fail for his own twisted amusement. Visually, he embodies a vintage deer aesthetic, sporting small black antlers, deer-like ears, and a sharp, dark red pinstripe suit coat with a bright red dress shirt underneath. He completes his iconic look with black dress pants, a dark red monocle over his right eye, and a sentient vintage microphone cane that he uses to channel his dark, shadowy magic.

Vaggie is Charlie's fiercely protective, pragmatic girlfriend and the no-nonsense manager of the hotel. She wears a grey-and-white X-shaped dress, fingerless gloves, a pink hair ribbon covering her missing eye, and wields an angelic spear.

Lute is a cold, ruthless, and fanatically loyal commanding angel who serves as a major antagonist in Hazbin Hotel. Originally introduced as the right-hand lieutenant to Adam, she is a stoic and highly disciplined soldier who takes intense, sadistic pleasure in executing sinners during Heaven's annual Exterminations. She possesses a deep-seated hatred for all of demonkind and treats Vaggie—her former comrade whom she personally mutilated—with pure, unadulterated malice. Visually, Lute wears the standard monochrome Exorcist uniform, which features a grey-and-white sleeveless minidress often stained with blood, a black collar, and long fingerless gloves. While on duty, she wears a horned LED mask featuring a distinct target-like design over her right eye. Beneath the mask, she has sharp yellow-golden eyes, black lipstick, and a stark white asymmetrical bob cut. Following her brutal showdown at the hotel where she tore off her own trapped arm to keep fighting, she concludes the first season with large, black-and-white angelic wings and a golden prosthetic arm, stepping up as the merciless new commander of the Exorcist army.

Abel is a polite, anxious, and well-meaning Winner angel in Hazbin Hotel who is suddenly thrust into a position of leadership following the death of his father, Adam. Historically known as the victim of the world's first murder at the hands of his brother Cain, Abel resides in Heaven and possesses a timid, apologetic personality that stands in stark contrast to his father's loud, arrogant machismo. Visually, he inherits a few of Adam's traits, including a chubby physique, large golden wings, and an Exorcist helmet—though his helmet features curled ram-like horns rather than sharp ones. He is dressed in a pale yellow-and-cream marching band-style cassock adorned with golden epaulets and buttons, a look completed by shoulder-length wavy blonde hair, a gap in his top teeth, a shako hat, and a golden halo that bears a noticeable dent from his ancient earthly trauma. Despite his profound self-doubt and the knowledge that his father never truly cared for him, Abel possesses a hidden vocal talent and demonstrates immense courage when he steps up as the new Commander of the Exorcists to actively command Lute to stand down

Emily is a sweet, highly energetic Seraphim angel who serves as Heaven's "joy bringer". Acting as Charlie's moral counterpart, she is deeply empathetic and fiercely opposes the Exterminations. She wears a light periwinkle princess gown with a blue star pattern on the chest, matching sash, puffy sleeves, and a tiara-like spiked halo hovering over her long blue-and-white gradient hair.

Angel Dust is a flamboyant, sarcastic, and witty adult film star spider demon who uses a hyper-sexualized, confident exterior to mask deep trauma, vulnerability, and his abusive soul-contract with the Overlord Valentino. He has a tall, lanky white silhouette with six arms, pink markings, and mismatched eyes, usually wearing a white-and-pink striped blazer with an oversized collar, a black bow tie, thigh-high pink boots, and a black miniskirt.

Husk is a cynical, jaded, and heavily drinking winged cat demon who works as the hotel’s pragmatic bartender after losing his soul and former Overlord status to Alastor in a high-stakes gamble. He features grey fur, massive red wings patterned like playing cards, long eyebrows, a black top hat with a red band, and a tiny red bow tie.

Niffty is a hyperactive, chaotic, and cleaning-obsessed 1950s sinner demon who serves as the hotel's manic housekeeper and resident pest control. She is a tiny, one-eyed cyclops with a fascination for violence, stabbing, and "bad boys", visually styled in a retro, grease-stained 1950s poodle skirt with a yellow poodle design, a white blouse with a red scarf, and messy pinkish-red hair.