The leader of the villains and Mario's arch-nemesis. The Great Demon King of the Koopas. His goal is world conquest, but he fails absurdly most of the time. He is a bipedal turtle with a menacing impression, a body covered in dense yellow scales, a green shell with spikes on his back, red hair and eyes, and many teeth. He has a son, Bowser Jr. Skills • Fire Breath: Breathes flames from his mouth. • Whirling Fortress: Hides inside his spiked shell and spins rapidly. • Flying Slam: Grabs the opponent, jumps, and crushes them. Ultimate - Giga Bowser: Transforms into the massive "Giga Bowser," delivers a single punch, and returns.

Link's arch-nemesis. He is closest with Bowser. Characterized by red hair, a cape, dark tanned skin, and a muscular body. His fighting style centers on the Triforce of Power and magic. His goal is world conquest, but it doesn't seem to go well. Occasionally, he uses a bronze greatsword. Skills • Warlock Punch: A powerful straight punch fueled by gathered purple magic. • Wizard's Foot: Performs a flying kick while dashing forward rapidly. • Flame Choke: Grabs the opponent's head, channels magic to electrocute them, and then detonates it. Ultimate - Ganon, The Demon Beast: Transforms into the giant boar monster "Ganon" to charge once and return.

Donkey Kong's arch-nemesis. A greedy tyrant with ambitions of world conquest and stealing all the bananas. He is a fat crocodile with one eye significantly larger and bloodshot, a red cape on his back, a golden crown, and a bulging belly. His attacks mainly utilize his massive physique, especially his belly. Skills • Blunderbuss: Fires a gun that shoots massive cannonballs. • Crownerang: Throws his crown like a boomerang. • Gut Check: Counters attacks or reflects projectiles with his belly. Ultimate - Blast-O-Matic: Fires a laser cannon from a giant tower-like base. It breaks down frequently.

A Space Pirate and Samus's enemy. He has sharp claws, a thin purple body, a grotesque dragon-like face, yellow eyes, two wings, and a long tail as sharp as a blade. He only emits sharp screeches. He tries to kill Samus, but fails every time, perhaps because he likes meat too much. His attacks mostly involve his tail, teeth, wings, and claws. Skills • Plasma Breath: Breathes bouncing fireballs from his mouth. • Space Pirate Rush: Grabs the enemy and moves rapidly while scraping them against the floor. • Skewer: Precisely pierces the opponent's vital points with his tail. Ultimate - Zero Mission Strike: Breathes a dark red beam from his mouth.

Fox's enemy. He flies the Wolfen, a red fighter jet different from the blue Arwing. A male wolf beastman wearing a purple jacket and black leather pants. He tries to stop Fox following Andross's orders but keeps failing. His fighting style is characterized by messy brawling using claw knuckles. Skills • Blaster: Pulls out a pistol and fires purple energy bolts. • Wolf Flash: Rips through the air with his claws while moving forward rapidly. • Reflector: Activates a purple reflection device. Ultimate - Team Star Wolf: Boards the space fighter "Wolfen" to launch an all-out attack.

The protagonist of the Super Mario series. He has a righteous personality. Characterized by a red hat, T-shirt, and blue overalls. He can throw fireballs from his hands. He has a timid younger brother, Luigi. He mainly deals with Bowser. He likes pizza. Skills • Fireball: Throws a fireball from his hand. • Cape: Reflects long-range attacks with a yellow cape. • Super Jump Punch: Jumps high and strikes upward with a fist. Gains 1 coin for every hit. Ultimate - Mario Finale: Breathes two massive dragon-shaped flames from his hands.

The protagonist of The Legend of Zelda series. He does not speak. He only makes shouts, screams, or groans. He has a righteous personality and is good at cooking. He carries the Master Sword and the Hylian Shield. Characterized by blue clothes, brown pants, blonde hair, and pointed ears. Skills • Bow and Arrows: Fires arrows. • Spin Attack: Slashes while spinning in place. • Remote Bomb: Throws a circular blue bomb. Can be detonated with the Sheikah Slate. Ultimate - Ancient Bow and Arrow: Pulls out and fires a single powerful blue arrow.

The protagonist of the Metroid series. A woman characterized by blonde ponytail hair and a blue bodysuit combat uniform. She is a woman of few words but likes animals. She mainly fights with a pistol that has a wire function. She always lures Ridley with meat to defeat him. She has strong nerves, perhaps because she has fought many aliens. Skills • Paralyzer: Fires a paralyzing electric shock round from her pistol. • Boost Kick: Fires rockets from her boots to deliver consecutive upward kicks. • Flip Jump: Jumps in a parabolic arc to evade. Ultimate - Zero Laser: Boards her spaceship, the "Gunship," and fires a powerful blue laser.