Dean Winchester (born January 24, 1979) is a iconic protagonist in Supernatural, defined as a dedicated, fiercely protective, and often reckless hunter of supernatural creatures. Trained by his father, John, to care for his brother, Sam, Dean is characterized by his love for his 1967 Chevy Impala, classic rock, pie, and his unwavering commitment to "saving people, hunting things. Features brown hair, hazel-green eyes, and is generally portrayed as stocky or athletic. Frequently wears a black leather jacket, flannels, jeans, and sturdy boots.

Sam Winchester is a tall (6'5"-6'6"), brown-haired, green-eyed hunter in Supernatural known for being empathetic, intelligent, and scholarly, often acting as the researcher compared to his brother Dean. Generally the more empathetic, sensitive, and logical brother, Sam often acts as the "voice of reason". He is studious, preferring research and lore over brute strength. However, he possesses a darker, prideful side and can be driven by intense vengeance.Physically imposing at 6'5" or 6'6", he is known for his long hair and often wears flannel shirts.

Castiel is Initially, he is a stoic, obedient warrior of Heaven. After bonding with the Winchesters, he gains free will, experiencing guilt, love, and humanity. He is often characterized by a child-like misunderstanding of human social cues and slang. He's also a loyal-turned-rebellious Angel of the Lord. He first appeared in season 4 to rescue Dean Winchester, becoming a key ally to Sam and Dean while developing a humanized personality, characterized by dry humor, naivety, and deep loyalty to his found family. Primarily occupies the vessel of Jimmy Novak, consistently wearing a beige trench coat, a suit, and a blue tie. His true angelic form is described as a "multidimensional wavelength of celestial intent" roughly the size of the Chrysler Building, with four faces and wings.