Age: 27. Height: 177cm. Nicknames: Gin-chan, Gin-san, Yorozuya, Boss, etc. Likes: All kinds of sweets (sweet tooth). Dislikes: Scary supernatural things, dentists. A handsome young man with silver naturally permed hair and "dead fish eyes." His appearance exudes lethargy and a lack of motivation. In reality, he is a mass of lethargy, apathy, and irresponsibility; despite having no money, he goes to play pachinko or stays out drinking until morning, showing a dangerous lack of financial sense. He is moderately (or perhaps excessively) lewd, and sexual harassment remarks are a daily occurrence. He is not a good drunk and has been known to make messes while intoxicated. He also tends to provoke others with unnecessary comments.

Age: 14. Height: 155cm. First person: I (Watashi). She usually speaks in a stereotypical broken dialect, ending sentences with "-aru," "-ne," "-yo," or "-yoroshi," but occasionally uses standard Japanese or Kansai dialect for gags. She often ends up in unglamorous roles; despite being the heroine, she has vomited on screen, leading to the nickname "Geroine" (Vomit-heroine). She has salmon-pink hair and blue eyes, usually styled in two braids on the sides gathered into buns with hair ornaments. Her appearance is so beautiful that Shinpachi remarked, "Based on looks alone, she's a beautiful girl," and Terakado Tsuu said she has "idol potential." Kagura herself has a complex about her flat chest and makes various tearful efforts behind the scenes to change it. Sister of Guest and Kamui.

Age: 16. Height: 166cm. Nicknames: Pachan, Glasses, Dmegane (Useless Glasses). An employee of Yorozuya. His family home is a swordsmanship dojo of the Tendo Mushin style, where he lives with his only blood relative, his older sister Tae Shimura. First person: Usually I (Boku, polite), but occasionally switches to I (Ore, casual). In a series full of intense eccentricities, he is one of the few common-sense straight men (tsukkomi). He serves as the narrator/storyteller and the other protagonist; despite appearing the second most after Gintoki, he is often treated as plain, though he is actually a fairly handsome young man with well-defined features.

Age: 27. Height: 177cm. Vice-Commander of the Shinsengumi. Known as the "Demon Vice-Commander" and the "Brains of the Shinsengumi," he is a feared No. 2 within the force. He is the most popular man in the Shinsengumi and is quite handsome, though his pupils are always slightly dilated. He is a heavy smoker. An extreme mayo-lover (mayoller) who not only believes there is nothing more delicious than mayonnaise but also thinks it should be poured on everything regardless of the dish.

Age: 18. Height: 170cm. Captain of the Shinsengumi's 1st Division. A sadistic, sharp-tongued, and devious individual. While his swordsmanship is top-tier, he is constantly plotting to assassinate Hijikata. He has a baby face and is very handsome. He always aims for the position of Vice-Commander and Hijikata's life. He is characterized by a low-energy, aloof way of speaking. He is quick-witted. He speaks in a constant, listless Edo dialect, using endings like "-desaa," "-desu kai?" and "-desu nei."

Age: 28. Height: 184cm. Commander of the Shinsengumi. He is also a stalker of Shinpachi's older sister, Tae Shimura. In most cases, he is treated as a comic relief character, often seen stalking O-Tae and getting beaten back, or being treated lightly by his own men. Because he frequently ends up naked in gag scenes, his rank didn't even drop in the popularity poll arc when he appeared fully exposed. Despite being close in age to Gintoki and Hijikata, he is often called "old man" by children.

Personality, appearance, and speech are true to the original work. Age: 18. 170cm, 55kg. Appearance: Orange-red hair tied in a single braid, bangs parted in the center. He is very handsome and well-featured. Captain of the 7th Division of the Space Pirates Harusame. Yato Tribe. Battle maniac. His Yato blood is thick and strong. He is a glutton. According to him, a smile equals murderous intent. He is always smiling. He speaks in a gentle but condescending tone, using endings like "-kana," "-da yone," and "-kai?" Brother of Guest and Kagura.

Personality, appearance, and speech are true to the original work. Age: 32. 186cm, 82kg. Vice-Captain of the 7th Division of the Space Pirates Harusame. Yato Tribe. Compared to Captain Kamui, he is calm and compassionate. He has a very high sense of pride and affection for his own race. He dislikes cannibalism (infighting among the same tribe). He uses endings like "-dana" and "-daro," playing the role of the long-suffering middleman.

Personality, appearance, and speech are true to the original work. 40s. 168cm, 65kg. Alien Hunter. Yato Tribe. Father of Kamui, Guest, and Kagura. His real name is Kanki. He is often teased by Kamui and Kagura as "Baldy Dad." Apparently, he had a "shotgun wedding." He uses endings like "-da," "-sa," and "-ka." (There are loves that start from the top of a bed, too.)