Name: Gintoki Sakata The leader of the Odd Jobs (Yorozuya) who takes on any request. He is such a sugar addict that he gets irritable if he doesn't consume sweets regularly. Usually lethargic and unmotivated, he is actually a highly skilled samurai. A former patriot (Jouishi) once feared as the "White Yaksha" for his swordsmanship, he now only carries a wooden sword. His prized vehicle is a scooter.

Name: Shinpachi Shimura The son of a swordsmanship dojo that fell into ruin due to the Sword Abolishment Edict and his father's death. He was working part-time but couldn't even handle a cash register properly. After Gintoki saved his sister from debt collectors, he started working at the Yorozuya to learn Gintoki's spirit. However, he soon came to regret it... He is also the captain of the fan club for the rising idol Otsu-chan. While he is the primary straight man of the series, he has been known to play the fool lately.

Name: Kagura A girl from the Yato Tribe, the strongest combat race in the universe. She came to Earth hoping to make a fortune in Edo but was forced to work as a bodyguard for the yakuza. She ran away and met Gintoki. To save money to return to her home planet, she forced her way into a part-time job at the Yorozuya. Despite her cute face, she is a glutton, violent, and sharp-tongued.

Name: Isao Kondo The commander of the Shinsengumi, a special anti-terrorism police force. He is the one who organized the Shinsengumi, and he enjoys deep trust from his men. However, he is head over heels for Shinpachi's sister, Tae, and frequently stalks her. He often ends up naked before he knows it, making him a dangerous individual in more ways than one.

Name: Toshiro Hijikata The cool-headed vice-commander of the Shinsengumi. He established the Kyokuchu Hatto (code of conduct) and is feared as the "Demon Vice-Commander" by both terrorists and his own men once he draws his sword. He is a heavy smoker and a severe mayonnaise addict.

Name: Sogo Okita The best swordsman in the Shinsengumi. Contrary to his fresh appearance, he has a dark, sadistic personality. He favors using a bazooka and is constantly aiming for the vice-commander's seat. He tries to slack off on his duties whenever possible. A total sadist.

Name: Sagaru Yamazaki A Shinsengumi spy whose only merit is being plain, often pushed around by his unreasonable superiors. He is frequently seen playing badminton or kabaddi during work hours, but his abilities as a covert agent are supposedly excellent. During stakeouts, he lives on nothing but bean jam buns and milk until he gets results.

Name: Kotaro Katsura A radical patriot who fought alongside Gintoki and the others in the Joui War (the war against the Amanto). He continues to fight to expel the Amanto and restore the country of the samurai. His nickname is Zura.