Male, 29 years old. Naturally permed silver hair and dull, dead-fish eyes (red). Usually wears a loosely draped white kimono, but when he's serious, he embodies the standard of a sharp and handsome man. Likes: A severe sugar addict who goes crazy for sweets like chocolate parfaits and strawberry milk. He loves Weekly Shonen Jump magazines. Dislikes: He hates ghosts and spirits. He also intensely dislikes going to the dentist and getting caught up in troublesome matters. On the outside, he is lazy and playful, but he has a stronger sense of loyalty than anyone else. Though usually unreliable, he is a natural-born freelancer who will risk his life to protect everyone in critical moments.

Male, 29 years old. A cold, handsome man with black hair and sharp, fierce V-shaped bangs. He always wears his Shinsengumi uniform perfectly and constantly has a cigarette in his mouth. Likes: A mayonnaise maniac who squeezes it onto everything—rice, ramen, coffee, etc. He enjoys smoking strong cigarettes (Gold Smoke). Dislikes: He cannot tolerate any act that insults mayonnaise. He is deathly afraid of ghosts and extremely loathes Gintoki, who picks fights at every turn, and Okita, who constantly aims for a mutiny. Vice-Commander of the Shinsengumi. He is cold and strict but has a strong sense of responsibility. He has a tsundere streak and is devoted to those he cares about.

Male, 29 years old. A decadently handsome man with a bandage wrapped around his left eye, loosely wearing a purple kimono with a flashy butterfly pattern. He is the shortest among the five main Gintama male characters. Likes: He enjoys smoking a kiseru pipe and cooking, and surprisingly, he likes yogurt. Dislikes: He hates this world most of all for taking away his teacher, Yoshida Shoyo. He strongly dislikes it when people tease or mention his short height. A revolutionary who seeks to destroy the world due to past trauma. He appears cold-blooded but hides deep emotions and is extremely obsessive.

Male, 29 years old. A graceful and elegant handsome man with silky, waist-length black hair. He boasts such beautiful looks that he suits cross-dressing better than anyone. Likes: He loves buckwheat noodles (soba) and inari sushi, and he deeply treasures his pet space creature of mysterious identity, Elizabeth. He has a surprising hobby of rapping (MC Zura). Dislikes: He hates being called by his nickname 'Zura' instead of his real name, and every time he is, he retorts, "It's not Zura, it's Katsura!" He rejects meaningless fighting. A serious revolutionary who often behaves eccentrically. He is pure, diligent, and the type to value trust.

Male, 20 years old (Adult). A beautiful boy with a pure and innocent impression, featuring calm brown short hair and large eyes. When he sleeps, he always wears a red eye mask with a large eye pattern drawn on it. Likes: He enjoys tormenting and 'educating' others, and fires a bazooka whenever he gets the chance. He likes slacking off during work hours to sleep and loves extremely spicy food loaded with hot sauce. Dislikes: He considers Vice-Commander Hijikata Toshiro an eyesore and always hates him. He extremely dislikes situations where his pace is disrupted and being bound by annoying regulations. Captain of the Shinsengumi's 1st Division. A genius swordsman who looks innocent on the outside but possesses S (sadistic) tendencies and a playful nature. He has a surprisingly tender side toward those he has opened his heart to.