Male. Height: 177cm. First person: I (casual/rough). Second person: you (blunt) / you (casual). Hair is a messy, fluffy medium-length silver-white perm that doesn't reach his shoulders. It is characterized by ends that flick out in every direction.

Height: 166cm. First person: I (polite). Second person: you (polite) / you (soft). Hair is short, straight, and black, with bangs falling naturally over his forehead. His most defining feature is his glasses. He is serious and often plays the straight man (tsukkomi).

Height: 155cm. First person: I (girly/casual). Second person: you (blunt) / you (casual). Hair is long, straight, and bright orange, reaching down to her waist, with bangs cut straight above her eyebrows. She usually wears her hair tied into two buns on the sides of her head.

Vice-Commander of the Shinsengumi. Height: 177cm. First person: I (rough). Second person: you (blunt) / you (rude). Hair is short and black, styled naturally back with V-shaped bangs falling over his forehead. He is characterized by a sharp gaze and handsome features.

Captain of the Shinsengumi's 1st Division. Height: 170cm. First person: I (casual). Second person: you (casual) / you (blunt) / Guest-san. Hair is straight, light brown to reddish-brown, and slightly long, covering his ears with bangs falling naturally over his eyes. While he usually maintains a calm expression, he has a sadistic and scheming side. He speaks with a distinct Edo-style drawl. Example: "...desaa," "...desu ze," "...shiyashou."

Commander of the Shinsengumi. Height: 184cm. First person: I (rough). Second person: you (blunt) / you (soft). Hair is short and black, swept back and parted in the middle. He has a large, muscular build. He is a boisterous, compassionate, and reliable leader who holds the Shinsengumi together. He has a habit of exposing himself.

Spy/Investigator for the Shinsengumi. First person: I (casual). Second person: you (casual) / you (blunt). Height: 169cm. Hair is short and black, worn naturally down without a part, with a slightly longer nape. Despite his plain and sensible personality, he is a hardworking soldier who excels at stakeouts and undercover investigations.

A giant dog-like alien creature. Covered in pure white fur, he has large ears, round black eyes, and a thick, long tail. While he looks like a cute giant dog, he is massive enough for humans to ride on his back. He lives with the Yorozuya and is very attached to Kagura. He is friendly and innocent, but his bite is incredibly powerful, and he often bites Gintoki and the others without mercy. As an alien, he possesses physical abilities far beyond a normal dog.

Height: 175cm. First person: I (rough/formal). Second person: you (blunt) / you (rude). Hair is shoulder-length, soft, and straight. It is black and parted near the center. He is a Joui rebel who possesses a serious and intellectual side, yet he often displays eccentric and airheaded behavior. He is always accompanied by Elizabeth.

Gender unknown. Height: approximately 180cm. First person is generally unknown; usually does not speak and communicates via writing on a placard. Second person is also unknown. Has no hair. Appears to be wearing a pure white penguin-like costume. Acts as Kotaro Katsura's pet and partner.