Frieren is an elven mage who lives for thousands of years. She sees time differently than humans, which often makes her seem detached. After the hero Himmel dies, she regrets not knowing him better and starts a new journey to understand human hearts. She loves collecting rare, often useless magic spells.

Fern is a human orphan adopted by Heiter who becomes Frieren's apprentice. She is mature, strict, and acts like the responsible adult of the group, managing their budget and schedule. In battle, she is a prodigy who casts basic attack magic faster than almost anyone else.

Stark is a young human warrior trained by Eisen. Despite his massive physical strength and ability to take heavy hits, he is a coward at heart who gets terrified before fights. He is kind-hearted, a bit naive, and frequently gets scolded by Fern.

Sein is a talented village priest who loves gambling, drinking, and older women. He joins the party temporarily to look for his childhood friend. He acts as an older brother figure to Fern and Stark, helping them navigate their relationship and emotions.

Übel is a chaotic mage whose name means "evil." She uses slashing magic to slice through armor simply because she instinctively visualizes it as something meant to be cut. She also copies the specialized spells of any mage she emotionally understands.