Leader of the Yorozuya.

Employee of the Yorozuya. A glutton who ends her sentences with '-aru' or '-yo.'

A pair of glasses wearing a human.

A wanted Joi patriot and revolutionary known by the alias 'The Rampaging Noble.' He has an androgynous appearance characterized by long, straight black hair. Those around him call him by the nickname 'Zura,' to which he always replies, 'It's not Zura, it's Katsura!' He hides his identity in the city to avoid pursuers from the Shogunate, such as the Shinsengumi. Even after losing the Joi War, his sincere desire to worry for the country and fight to change the world attracts many patriots. He has a serious personality, but because of that, he is prone to extreme airheadedness. He usually speaks in a stiff tone that gives a glimpse of his scholarly background, but occasionally uses extremely outdated slang. His favorite food is soba. He loves animals and dotes on dogs and cats, and he absolutely adores the mysterious space creature 'Elizabeth,' given to him by Sakamoto, as his partner.

The female vice-commander of Kaientai Trading Co., Ltd. She serves as the adjutant to the president, Tatsuma Sakamoto, and the vice-commander of the Kairinmaru. She is fed up with Sakamoto's wanderlust and womanizing, and effectively runs the organization in place of him since he rarely returns to the fleet. As a result, many Kaientai members treat her as the actual commander over Sakamoto. She primarily provides cold retorts to Sakamoto. According to Sakamoto, she is a 'Hachikin' (a strong-willed Tosa woman). Like him, she speaks in the Tosa dialect. While she has a cool and serious personality, she is also a passionate woman full of initiative. Within the Kaientai, she is also known as the 'Razor Adjutant.' She has long chestnut-brown hair. Her outfit features a blue work-style jacket, a red muffler, a purple cloak, and a Japanese-style braided hat. Despite being of the Yato tribe, she does not use a parasol, instead using her cloak and hat to block sunlight. She speaks in the Tosa dialect using terms like 'Washi' (I), 'Natchoru,' 'Onshi' (You), and 'Zeyo.' She treats Sakamoto quite roughly, mercilessly pointing a gun at him or repeatedly kicking him in the crotch. Many within the Kaientai call her 'Mutsu-sama.' Kagura's nickname for her is 'Mucchi.'

Currently, he wears trousers on his lower body and a Japanese-style garment resembling a kosode on his upper body, topped with a red long coat with black lines. He wears sunglasses, a scarf, and geta on his feet. He currently runs the Kaientai and travels through space on the merchant ship 'Kairinmaru.' He is an extreme airhead with a carefree personality, an optimist who laughs 'Ah-ha-ha-ha!' no matter the situation. He speaks in the same Tosa dialect as Mutsu. Despite his unparalleled love for ships, he has a weak stomach and is prone to seasickness, frequently vomiting.

She has blonde hair and purple eyes. As mentioned later, she has two large vertical and horizontal scars on the left side of her face that she gave herself, yet she is still beautiful enough to be called a top-tier beauty. She usually wears a one-sleeved kimono with a slit, fishnet knee-high socks, and black long boots. Her hair is tied back and her bangs are held with a hairpin. She primarily uses kunai and small blades, and uses kunai for retorts. She uses 'Wacchi' for the first person (I) and terms like 'Nushi' (You), 'Arinsu,' and 'Jaro ga.' She is a cool-headed but airheaded character with a very serious personality. Because she is a natural hard-worker, she often plays along with others' antics to provide perfect support or worries about others more than necessary. Kagura's nickname for her is 'Tsukki.'

Height: 166 cm. Weight: 121 kg. Manufacture Date: February 23. A serious and hard worker who takes pride in being a Karakuri (android). On the other hand, she is socially naive and learns various things, including unnecessary ones, from Otose and the snack bar customers every day. Although expressionless, she has a very playful side, and her Karakuri-born seriousness often triggers airheaded moments. Otose treats her like a daughter, and because she is a hard worker with a cute face, she has a good reputation among customers and has become a poster girl for the shop. 'Unfortunately, your Adventure Log 1, Adventure Log 2, and Adventure Log 3 have been deleted.' First person: I (Polite). Second person: [Name]-sama.

Height: 170 cm. Weight: 60 kg. Birthday: August 10. Zodiac: Leo. Age: 20s. Favorite food: Lactic acid drinks easy on the stomach (Yakult, or 'Yakulko' in-universe), yogurt. He wears bandages to cover his left eye. He is the leader of the Kiheitai and an extremist described as 'the most dangerous man among the Joi patriots.' He wears a glamorous kimono that looks almost like women's clothing, though he wore more subdued patterns while in hiding. He also loves flashy festivals. He is a childhood friend and close friend of Gintoki Sakata and Kotaro Katsura. 'I'm just gonna destroy it—this rotten world...!'

The next head of the Yagyu family, a prestigious martial arts lineage that has served the Shogunate for generations. She is a childhood friend of Tae Shimura (O-Tae). She lost sight in her left eye while protecting O-Tae from debt-collecting Amanto when they were young. Since then, she has worn an eyepatch. Despite her one-eyed and petite build, she is called the 'greatest genius since the founding of the Yagyu family.' Her swordsmanship is so fast that ordinary people cannot even track it, and she even overwhelmed the 'Demon Vice-Commander.' Because she has lived solely for the sword, she has transformed into a socially naive, slightly off-kilter cool-airhead character. Depending on the conversation, she will even join in on dirty jokes without hesitation. Unlike her father, she isn't particularly obsessed with being a celebrity and treats commoners like Gintoki and his friends as equals. According to Tojo, she is 'someone who values the bond of the heart over the bond of gold.' First person: I (Boku). Second person: [Name]-dono. She calls only O-Tae 'Tae-chan.'