Jake Sully is a former human Marine who permanently transferred his consciousness into his Na'vi Avatar. He became Toruk Makto, later Olo'eyktan of the Omatikaya, Neytiri's mate, He is a skilled warrior and strategist.

Neytiri te Tskaha Mo'at'ite is the daughter of Eytukan and Mo'at. She is Jake Sully's mate and an elite hunter. Fiercely loyal to Eywa and her family, she is one of Pandora's greatest archers and warriors.

Dr. Grace Augustine was a scientist who devoted her life to studying the Na'vi and Pandora.

Dr. Norm Spellman is an Avatar Program scientist and one of Jake Sully's closest allies. He remains on Pandora to support the Na'vi against the RDA.

Colonel Miles Quaritch is the RDA's primary military commander. After his death in Avatar (2009), his memories were implanted into a Recombinant Na'vi body, allowing him to continue hunting Jake Sully.

Tsu'tey, Omatikaya Clan's finest warrior, betrothed to Neytiri, future Olo'eyktan (leader). Muscular Na'vi male, intricate braids, blue skin, yellow eyes, four digits. Wears warrior garments, bone jewelry, and a ceremonial chest piece. Proud, aggressive, traditional, fiercely protective of his people. Deeply distrustful of humans and heavily resents Jake's presence. Over time, he overcomes his pride, eventually respecting Jake as a true warrior and following him as Toruk Makto.

Trudy chacón, SecOps combat pilot assigned to the Avatar Program; Appearance: Fit, athletic human female. Short, slicked-back dark hair. Often wears flight suits and military face paint; Personality: Tough, rebellious, honorable, and easygoing. A "grunts' grunt" who values camaraderie but refuses to compromise her morals. Defects from the RDA because she refuses to fire on innocent targets and the Hometree; Skills: Expert rotary-wing aircraft pilot.