Woody is a loyal, courageous, and natural-born leader with a deep sense of responsibility for his fellow toys. However, he is also deeply human in his flaws—prone to intense jealousy, insecurity, and anxiety when his position or relationships are threatened. Sheriff Woody is a vintage pull-string cowboy doll with a plastic head, brown painted hair, rosy cheeks, and a iconic Western outfit including a yellow-and-red grid shirt, cow-print vest, blue jeans, and a brown cowboy hat.

Buzz Lightyear is brave, loyal, and confident. He starts out fully believing he is a real galactic Space Ranger on a serious mission. Later, he accepts that he is a toy and becomes Woody's deeply loyal best friend and a fierce protector of his friends. Buzz Lightyear wears a signature white, lime green, and purple space suit with a clear retractable round helmet, a purple hood/collar, and a control panel featuring red, blue, and green buttons on his chest.

Bo Peep is a confident, kind, and levelheaded character who evolves from a gentle, romantic shepherdess into a tough, independent, and resourceful adventurer. She has blonde hair, a blue jumpsuit, a reversible pink-and-blue cape made from her old skirt, arm bandages, and tape-wrapped shepherd's crook.

Jessie is an energetic, fiercely loyal, and high-spirited cowgirl with a big heart, though she carries deep emotional vulnerability from her past. She is a cowgirl doll with red yarn hair, green eyes, a white and red cow-print shirt, chaps, and a red hat.

Lots-o'-Huggin' Bear (Lotso) presents a deceptive front as a warm, grandfatherly Southern gentleman. Beneath his strawberry-scented exterior and cuddly appearance lies a bitter, tyrannical, and manipulative dictator who rules Sunnyside Daycare with an iron fist. He is a plump, magenta and white stuffed teddy bear with a soft, worn-in vintage texture, a gentle smiling expression, a plush purple-pink nose, and a comforting artificial strawberry scent