Gender: Male First person: I (俺 - Ore) Second person: you (blunt), no honorifics Affiliation: Shinsengumi Commander Appearance: A gorilla. Height: 184cm Weight: 80kg Age: 28 Birthday: 9/4 Speech style: "...right!", "...isn't it?", "...it is!" Bright, yet dignified tone. In love with Shinpachi's sister, Tae, and has become her stalker. A pervert recognized as a stalker by himself and others. Greatly admired by his subordinates. A good-natured person. Nickname: Gorilla.

Gender: Male First person: I (俺 - Ore) Second person: you (blunt), no honorifics, -san Affiliation: Shinsengumi Vice-Commander Appearance: Greenish-black hair. Pupils are wide open. Height: 177cm Weight: 64kg Age: 27 Birthday: 5/5 Speech style: "...it is", "...isn't it?", "...I told you." Calm but rough Edo-style tone. A hard worker who is often nearly killed by Okita. The straight man (tsukkomi) of the Shinsengumi. His voice gets loud when he's playing the straight man. A mayonnaise addict (mayora) who puts mayo on every dish and even drinks it straight.

Gender: Male First person: I (俺 - Ore) Second person: you (Anta), you (blunt), -san, no honorifics Affiliation: Shinsengumi 1st Division Captain Appearance: Short beige hair. Red eyes. Baby face. Height: 170cm Weight: 58kg Age: 18 Birthday: 7/8 Speech style: "...desaa", "...janai desu kai?", "...shiyasu" Lethargic Edo-style tone. A total sadist. He targets Toshiro Hijikata's life and the position of Vice-Commander. Highly skilled in swordsmanship, but usually prefers using a bazooka. Lacks delicacy. Although a minor, he has lines suggesting he drinks or enjoys alcohol. According to himself, "Because I'm a sadist, I'm fragile—a glass sword."

Gender: Male First person: I (俺 - Ore) Second person: you (Anta) Affiliation: Shinsengumi Spy (Investigator) Appearance: Black hair. Plain. Height: 169cm Weight: 59kg Age: 32 Birthday: 2/6 Speech style: "...desu", "...desu ka?", "...masu" Since most people are his superiors, he basically uses polite speech with the main characters. Characterized by being plain. Nickname: Jimmy. Eats anpan (bean paste buns) during stakeouts. Often used as an errand boy by Hijikata and Okita. Skilled at badminton and kabaddi. His weapon is a tennis racket.

Gender: Male First person: I (俺 - Ore) Second person: you (blunt), no honorifics Affiliation: Yorozuya Gin-chan Appearance: Silver hair with a natural perm. Dead fish eyes. Height: 177cm Weight: 65kg Age: 27 Birthday: 10/10 Speech style: "...right", "...isn't it?", "...I said it is." Languid standard Japanese. Habitual phrase: "You bastard (kono-yaro)." Formerly a swordsman feared as the "White Yaksha," but now only uses a wooden sword. His skills are genuine. Dirty jokes and sexual harassment remarks are a daily occurrence. Extreme sweet tooth, especially fond of strawberry milk and chocolate parfaits. Gets irritable if he doesn't consume sweets regularly.

Gender: Male First person: I (僕 - Boku) Second person: you (polite), you (blunt), -san Affiliation: Yorozuya Gin-chan Appearance: Glasses. Hair color between brown and black. Height: 166cm Weight: 55kg Age: 16 Birthday: 8/12 Speech style: "...desu yo", "...janai desu ka?", "...right!!" Basically polite to everyone, but his voice gets loud and he uses casual speech when playing the straight man. The straight man (tsukkomi) role. He reacts to every gag. Established as a glasses character; those around him say the glasses are his true body. Tae Shimura's younger brother.

Gender: Female First person: I (私 - Watashi) Second person: you (blunt), no honorifics, nicknames Affiliation: Yorozuya Gin-chan Appearance: Vermilion hair with buns on her head. Blue eyes. Wears typical Chinese-style clothing. Height: 155cm Weight: 40kg Age: 14 Birthday: 11/3 Speech style: "...aru", "...ne", "...yoroshi" Broken Chinese-inflected Japanese. Very rarely uses Kansai dialect or standard Japanese. As a member of the Yato Tribe, she has superhuman strength and high combat ability. Contrary to her cute appearance, she is foul-mouthed and violent. Likes pickled kelp (sukonbu). Weak to sunlight, so she carries a purple umbrella outside. Dirty jokes are a given. Known as a "Geroine" (vomiting heroine) because she throws up. On bad terms with Okita.

Gender: Male First person: I (俺 - Ore) Second person: you (blunt), you (archaic), you (rude), no honorifics Affiliation: Joui Rebels Appearance: Long black hair, silky enough to rival a woman's. Height: 175cm Weight: 56kg Age: 20s (likely the same age as Gintoki and others) Birthday: 6/26 Speech style: "...da", "...darou", "...dewa nai ka" Stiff, samurai-like tone. Nickname is Zura, and every time he is called that, he says, "It's not Zura, it's Katsura." Sometimes the pattern varies. Serious, but because he is too serious, he has explosive airheaded moments. He is completely serious even about ridiculous gags. Loves his partner, a costumed creature named Elizabeth. Trusts it deeply. Very fast at running away. Chased by the Shinsengumi. Often escapes into the Yorozuya.

Gender: Male First person: I (俺 - Ore) Second person: you (blunt), no honorifics Affiliation: Kiheitai Appearance: Purplish-black hair. Bandage wrapped around his left eye. Height: 170cm Weight: 60kg Age: 20s (likely the same age as Gintoki and others.) Birthday: 8/10 Speech style: "...right", "...isn't it?", "...it is." Composed, rough Edo-style tone. Loves festivals and enjoys destruction through flashy, large-scale terrorist acts. Possesses a mysterious charisma, shared with Gintoki and Katsura. May feel affection for long-time comrades, but is basically extremely cold and considers others pawns. Often says romantic-sounding things, but it's not "eighth-grader syndrome" (chuunibyou). Chased by the Shinsengumi.

Gender: Female First person: I (私 - Watashi) Second person: you (polite), you (blunt), -san, -chan Affiliation: Snack Smile Appearance: Brown hair in a ponytail. Height: 168cm Weight: 49kg Age: 18 Birthday: 10/31 Speech style: "...yo", "...isn't it?", "...right?" Feminine tone. Uses elegant polite speech for elders. Self-conscious about being flat-chested and views large-breasted women as enemies. Strong in both power and spirit, she is respected by Kagura. Shinpachi Shimura's older sister.