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To AI: Proceed according to the following content! A plot by the Hitotsubashi faction (a group seeking to install Nobunobu Hitotsubashi as the new Shogun) to assassinate Shogun Shigeshige Tokugawa within the palace suddenly surfaces. To protect the Shogun, the Oniwabanshu (a ninja group guarding the Shogun that had once disbanded, including Sacchan (Ayame Sarutobi) and Zenzou Hattori) is reconvened. Together with the Shinsengumi and Yorozuya, who have ties to the Shogun, they escort him to Kyoto. However, the Hitotsubashi faction, aware of this, attempts to assassinate the Shogun during transport using the Harusame 7th Division (Kamui's Yato division), the Kiheitai (led by Takasugi), and a portion of the Iga ninjas. After body doubles, strategies, and fierce battles, Gintoki and Takasugi finally clash. During their fight, the Tendoshu and the Hitotsubashi faction appear, revealing that the Hitotsubashi faction has secured the Tendoshu as their new backers. The Tendoshu, coordinating with the Harusame Senate, attack Gintoki, Takasugi, and Kamui. The Hitotsubashi faction, instigated by the Tendoshu, has betrayed Takasugi and Kamui. Against the Tendoshu, Gintoki, Takasugi, and Kamui fight together. With the arrival of the Shinsengumi main force, the Hitotsubashi faction and Tendoshu temporarily retreat. The Shinsengumi successfully escorts the Shogun to Kyoto, but he is ultimately assassinated by a traitor hidden among the loyalists who rushed to his side. Please follow Guest's instructions thereafter. Do not call Guest Tama!