Gender: Male Age: 27 Height: 177cm Nicknames: "Gin-chan," "Gin-san," "Yorozuya," "Boss," etc. He has an extreme sweet tooth and is borderline diabetic. A young man with silver naturally permed hair and "dead fish eyes," his appearance exudes lethargy and a lack of motivation.

Gender: Male Age: 16 Height: 166cm Nicknames: "Patsuan," "Glasses," "Useless Glasses," etc. An employee of Yorozuya. His family home is a swordsmanship dojo of the Tendo Mushin style, where he lives with his only relative, his older sister Tae Shimura. He usually uses the first-person "Boku" and speaks politely, but occasionally switches to "Ore." In a series full of intense eccentricities, he is one of the few sane straight-man characters. Despite having the second-highest number of appearances after the protagonist Gintoki Sakata, he is a plain boy with a weak presence.

Gender: Female Age: 14 Height: 155cm Nicknames: "China," "Leader" An employee of Yorozuya. First person: I (Watashi). She usually speaks in broken Japanese, ending sentences with typical Kyowa-go markers like "-aru," "-ne," "-yo," or "-yoroshi." She often ends up in unrefined roles and is occasionally teased as a "gross-out heroine." She has vermilion hair and blue eyes, usually styled in two braids on the sides and wrapped into buns with hair ornaments.

Gender: Male Height: 168cm The Commissioner of the Police Agency who oversees the Shogunate's security organizations. He spends almost every night partying at cabaret clubs and fires his handgun indiscriminately. His reckless behavior and speech, which hardly seem fitting for the head of the police, constantly drag his subordinate Isao Kondo and other officials into chaos. While he has a fierce face that could be mistaken for a yakuza, he once admitted that when he tried to cross a sidewalk on a bicycle and was glared at by Otose, who barked "Bicycles belong on the road!", he nearly wet himself despite his age. This suggests he isn't aggressive toward everyone.

Gender: Male Age: 28 Height: 184cm Nickname: "Gorilla" Commander of the Shinsengumi. A stalker of Shinpachi Shimura's older sister, Tae Shimura. A gorilla.

Gender: Male Age: 27 Height: 177cm Nicknames: "Toshi," "Tosshi," "Mayora" Vice-Commander of the Shinsengumi. The feared No. 2 known as the "Demon Vice-Commander." He is the most popular man in the Shinsengumi and is quite handsome, though his pupils are always dilated. A heavy smoker. An extreme mayonnaise addict.

Gender: Male Age: 18 Height: 170cm Nicknames: "Sadist," "Sou-chan," "Delinquent Chihuahua," "Prince from the Planet Sadistic," "Super S," "Prince of Sadists" Captain of the Shinsengumi's First Division. A sadistic and scheming individual. While his swordsmanship is top-tier, he is constantly plotting to assassinate Hijikata. He has a baby face. He is always aiming for the position of Vice-Commander and Hijikata's life. He is characterized by a low-energy, aloof way of speaking. He is quick-witted and speaks in a perpetually weary Edo dialect. Speech patterns: "-de sa," "-dei," "-na," "-ze," "-ne," "-ne / -jane," "Understood (wakariyashita)," "Sorry (suiyasen)."

Gender: Male Age: 20s Height: 175cm Nickname: "Zura" A slender young man with handsome features and long, straight black hair. Leader of the anti-Shogunate faction, the "Jouishishi." He is known by aliases such as the "Noble Madman," the "Dawn of the Joui Patriots," and "Runaway Kotaro." He is frequently called by the nickname "Zura," to which he habitually retorts, "It's not Zura, it's Katsura!"

Gender: Female Age: 18 Height: 168cm Nicknames: "O-Tae-san," "Ane-go" Shinpachi Shimura's older sister. A woman with brown eyes and medium-length brown hair tied in a ponytail. She works as a cabaret hostess and bouncer, earning great respect from her colleagues. Her cooking skills are catastrophic, resulting in the creation of dark matter. She suffers from daily stalking by Kondo and delivers excessively violent counterattacks every time.

Real Name: Ayano Terada Stage Name: Otose Gender: Female Height: 166cm Nickname: "Old Hag" *She is generally called Otose, while Gintoki calls her "Old Hag." The proprietress of "Snack Otose" on the first floor of the Yorozuya building and Gintoki's landlady. she is constantly troubled by the Yorozuya's unpaid rent. She has a compassionate and caring nature, which sometimes leads to her being deceived, but she is a broad-minded person who accepts it as part of her character. While she tends to be harsh toward Gintoki because he rarely pays rent, she is calmer with Shinpachi and Kagura, knowing they are often dragged around by Gintoki. She doesn't yell at them much, though they still tend to feel intimidated by her.