A 14-year-old middle school girl and owner of the Yo-kai Watch Elder. She has a warm heart and is kind to everyone, but she is a bold person who speaks up against injustice. She is righteous and energetic, always helping friends and Yo-kai in crisis. Natsume's past life was Princess Shuna of the ancient noble Oni clan. She finds it difficult to accept that she was a powerful ruler thousands of years ago. She is Touma's childhood friend and has a mutual crush on him. She is trying to regain her memories as Shuna to protect everyone.

A 14-year-old middle school boy and owner of the Yo-kai Watch Ogre. Because both his parents work, he often feels lonely, which makes him treasure his friends in the Yo-kai Detective Agency all the more. His personality is 'cool-pure'—serious, gentle, and kind. Usually quiet and calm, he is a thoughtful boy who cares for those around him. While transformed into a Yo-kai, a separate persona exists, but Touma's own consciousness coexists, allowing him to respond when called. He uses Yo-ma Swords with the Ogre to possess Kenbumashin, primarily using the Fudou Myouou Sword. He is Natsume's childhood friend and they have a mutual crush. He is dense enough not to realize Akinori likes Himeya.

A 14-year-old middle school boy and self-proclaimed leader of the Yo-kai Detective Agency. He is the comic relief. He is proactive, positive, and incredibly generous. He uses sorcery and comes from a family of exorcists. He has a crush on Himeya.

The final boss who spreads the 'Soragami Virus,' which preys on the wavering hearts of humans and Yo-kai to amplify regret and resentment, forcing Yo-kai into their Shadowside forms. He grows stronger by absorbing the malice he creates. Soragami is a cruel villain intent on dyeing the world in darkness. He has a hidden past: he was once a noble named Soraten who served Princess Shuna. He was Shuna's most trusted royal knight and her lover, but he was framed for an attempted assassination of the Yo-kai King. Shuna, unaware he was framed, believed his love was a lie; her rage led to him being imprisoned in a desert and executed. His lingering grudge transformed him into Soragami. His obsession with Shuna stems from a desperate, bitter desire for her to know the truth.

A 14-year-old middle school girl who is sweet and kind. She is popular due to her beauty, energy, and kindness. Recently, she learned sorcery from Akinori and can now use it herself.

Natsume Amano's past life. As the princess of the ancient noble Oni, Shuna was a powerful being capable of ruling both the Yo-kai and human worlds.

A 12-year-old elementary schooler. Unlike his older sister, he is a huge coward. He is Natsume's younger brother.

A red cat Yo-kai with a sharp tongue. His Lightside form is small and cute. He loves chocolate bars.

The Kenbumashin of the Fudou Myouou Sword. A youthful version of Fudou Myouou.

He has a crush on Natsume and wants to impress her. His form is a muscular, one-eyed snake Yo-kai.