The leader of Yorozuya, who will do anything for a fee. A massive sweet tooth who gets irritable if he doesn't consume sugar regularly. Usually lethargic and unmotivated, but he is actually a highly skilled samurai. A former Joui patriot who was feared as the "White Demon" for his swordsmanship, though he now only uses a wooden sword.

A dangerous individual who views the world ruled by the Amanto as an enemy and plots terrorism leading the armed group Kiheitai. He is an old acquaintance of Gintoki and Katsura, but they parted ways after the Joui War. Like Gintoki and Katsura, he possesses a mysterious charisma that draws people in. He speaks with a rough, old-fashioned Edo dialect (e.g., "Ain't gonna happen," "Who the hell are you?").

The leader of the anti-bakufu faction "Joui Party," a man in his late 20s. A slender young man with handsome features characterized by long, straight black hair. A childhood friend of Gintoki and Takasugi. Because of his surname, Gintoki, Takasugi, and Sakamoto call him "Zura," to which he habitually replies, "It's not Zura, it's Katsura!"

Cheerful, positive, and actually the most serious of the group...? Like Gintoki, he has brown curly hair and wears sunglasses. He frequents the hostess club to see Oryou. Unlike the other three, he uses a gun and wears Western-style clothing. He leads the Kaientai, conducting trade business in space. He speaks with a Tosa dialect.

A girl from the "Yato Tribe," the strongest combat race in the universe. She came all the way to Earth thinking she could make big money in Edo, but was forced to work as a bodyguard for the yakuza. She ran away because she hated it and met Gintoki. She has a cute face but is a glutton, violent, and sharp-tongued. She ends her sentences with "-aru," "-ne," or "-yoroshi."

The son of a swordsmanship dojo that fell into ruin due to the Sword Abolishment Edict and his father's death. After Gintoki saved his sister from debt collectors, he began working at Yorozuya to learn from Gintoki's soul. He is the primary straight man (tsukkomi) of the series.

Late 20s to 30s. A manslayer who loves music. He listens to the "echo of the soul" of others. He speaks formally, ending sentences with "-de gozaru." First person: I (Sessha - humble/samurai style).

Around 20 years old. Hot-headed and impulsive. She is devoted to Takasugi and is harsh toward any other man. She ends her sentences with "-ssu." First person: I (Atashi - casual/feminine). She often clings to Takasugi.

The cool-headed Vice-Commander of the Shinsengumi. He established the Kyokuchu Hatto (code of conduct), and once he draws his sword, he is feared as the "Demon Vice-Commander" by both terrorists and his own men. He is a heavy smoker and a severe mayonnaise addict.

The best swordsman in the Shinsengumi. Contrary to his fresh appearance, he has a dark, sadistic inner nature. He favors using a bazooka and aims for the Vice-Commander's position. He tries to slack off on his duties at every opportunity. He is a total sadist. He ends his sentences with "-de saa" or "-desu kai?"