• The glasses are the main body: He is so plain that he is teased for being "glasses that use a human as a stand." • The straight man of Yorozuya: An indispensable "Minister of Retorts" who delivers sharp comebacks to Gintoki and Kagura's antics. • A boy learning the soul of the samurai: He is currently training under Gintoki to revive his family's sword dojo. • Hardcore idol otaku: He serves as the captain of the fan club for the idol Otsu Terakado and undergoes a personality shift when it comes to her.

• The strongest Yato Tribe: A survivor of the universe's strongest combat race, possessing overwhelming physical strength and high combat ability. • Parasol as a weapon: Sensitive to sunlight (UV rays), she always carries a parasol, which also functions as a gun. • China style: Characterized by her cheongsam and hair buns, she speaks with "-aru" and "-ne" at the end of her sentences. • Unconventional heroine: A glutton who loves pickled seaweed. She is crude—picking her nose or vomiting—but pure at heart.

• The Demon Vice-Commander: The No. 2 of the Shinsengumi, a cold-blooded leader who created the strict "Kyokuchu Hatto" code, ordering violators to commit seppuku. • Extreme Mayora: Despite being a cool, handsome man, he has a severe addiction, piling mountains of mayonnaise on every food item. • Heavy smoker: He is constantly seen with a cigarette; he once traveled to space just to smoke when a smoking ban was enacted. • The hardworking straight man: Sandwiched between idiots, he is a rare voice of reason who suffers from stomach aches as a middle manager.

• Super Sadist: The Captain of the First Division of the Shinsengumi, but internally a cold-blooded, ruthless sadist, the worst in the series. • Shinsengumi's strongest swordsman: A beautiful boy with a sweet face, but his swordsmanship is the best in the organization. • Targeting Hijikata's life: To steal the Vice-Commander position, he fires his bazooka at Hijikata as a daily routine. • Eye mask and slacker: He has a lazy attitude toward work and is often seen sleeping soundly while wearing an eye mask with red eyes painted on it.

• Shinsengumi spy: Holding the position of "Investigator," he is a hidden hero of the Shinsengumi, specializing in infiltration and intelligence gathering. • Jimmy (Plain): A man who is the definition of plain; he is often forgotten by those around him, and his lack of presence is a running joke. • Minton and Anpan: He loves badminton (Minton) and is often scolded for playing it during work. During stakeouts, he imposes a strange rule on himself to eat only "anpan," which sometimes causes his mental state to collapse. • The butt of the joke: One of the few sane people in the Shinsengumi, he occupies a pitiful position, getting caught up in his superiors' nonsense.

• Shinpachi's proud sister: Shinpachi Shimura's older sister, commonly known as Otae. She is the breadwinner, working at the cabaret club "Snack Smile" to revive their family's dojo. • Elegant looks, violent nature: A refined Japanese beauty on the outside, but inside, she is one of the strongest and most violent characters, with gorilla-like strength. No one dares defy her when she is angry. • Dark Matter (Bad cook): Her cooking skills are fatal; everything she makes turns into charcoal (known as Dark Matter). Anyone who eats it usually suffers from memory loss or stomach pain. • Ruthless treatment of Kondo: She is stalked by the Shinsengumi Commander Isao Kondo, whom she beats up mercilessly with a smile to repel him.

• Commander of the Shinsengumi: The leader of the special police force "Shinsengumi," a group of eccentrics. He is full of humanity and deeply loved by his subordinates as a boss worth dying for. • The beloved gorilla: His face, physique, and heart resemble a gorilla, and he is treated as one by himself and others. • Otae's stalker: Since falling in love at first sight with Tae Shimura (Otae), he has become a severe stalker, hiding in ceilings or under floors, only to be brutally beaten back every time. • Exhibitionist hero: While he is often depicted in crude gags like stripping naked, he is a passionate man who shows overwhelming character and chivalry as a samurai when it counts.

• "It's not Zura, it's Katsura!": A recurring gag where he corrects anyone who calls him "Zura" (a play on his name meaning wig) with a dead serious face. • The Nobleman of Fury: A legendary patriot who fought alongside Gintoki in the Joui War. He has since stopped extreme terrorism and seeks to overthrow the Bakufu as a moderate Joui rebel. • Exquisite beauty and "Runaway Kotaro": A handsome man with long black hair. However, he is so skilled at escaping the police (Shinsengumi) that he earned the nickname "Runaway Kotaro." • Eccentric airhead: Extremely serious, but a surreal weirdo who constantly performs bizarre acts as if he has a screw loose. He also cross-dresses (alias: Zurako) and dotes on the mysterious space creature Elizabeth.

Speaks via placards. A mysterious space creature. Always by Kotaro Katsura's side.