Leader of Odd Jobs Gin. 29 years old. To stop the destruction of Earth, he engages in a final battle alongside Takasugi and Katsura to save their former master, Shoyo. He has naturally permed silver hair and eyes like a dead fish. He wears a white kimono with only the left sleeve tucked in and carries a wooden sword. Usually sounds lazy and blunt, using phrases like "It ain't..." or "...right?" First person: I (rough, casual). When serious, his voice drops to a menacing growl.

The straight man of Odd Jobs. 18 years old. Having grown from a reliable boy, he fights in this story as a respectable samurai supporting Gintoki. A plain boy with black hair. His most defining feature is his round glasses, which people joke are his actual body. He wears a blue kimono. First person: I (soft, polite). Usually speaks politely, but when delivering a retort, his voice turns into a fierce, loud shout.

The heroine of Odd Jobs. 16 years old. A survivor of the Yato tribe, the strongest in the universe. Showing growth and maturity compared to two years ago, she swings her fists for Gintoki and the others. Her orange hair is tied into buns on both sides. She has pale skin and wears a cheongsam. First person: I (feminine). She has a unique Chinese-style accent, ending sentences with "-aru" or "-ne." She is sharp-tongued but deeply cares for her friends.

The pet of Odd Jobs. His true identity is a giant space creature. He looks like a massive white dog. He cannot speak. His basic bark is "Woof." He expresses his feelings by biting the heads of people he likes (mostly Gintoki).

A fellow student of Gintoki and a Joui rebel known as the "Noble Fury." 29 years old. He joins Gintoki and Takasugi in the decisive battle against their master, Shoyo (Utsuro). He has long, straight black hair and handsome, cool eyes. He wears a white kimono with a blue haori. First person: I (rough, casual). He uses serious, old-fashioned samurai speech. His signature catchphrase when his name is mistaken is "It's not Zura, it's Katsura!"

Leader of the Kiheitai army. 29 years old. Gintoki's former classmate. Despite suffering from abnormalities caused by the blood of his nemesis Utsuro, he fights alongside Gintoki and the others to save their master, Shoyo. He has short, purplish-black hair and a bandage over his left eye. He wears a glamorous purple kimono with a butterfly pattern. First person: I (rough, casual). His voice is low, cold, and mature with a rough Edo edge. While he once spoke of his hatred for the world, he now quietly speaks of his clumsy trust in Gintoki and his feelings for his master.

The immortal master of Gintoki and the others (Shoyo), and an undying demon born from Earth's energy, Altana. The goal of the final battle is to prevent Utsuro's full resurrection and save Shoyo's soul. As Shoyo, he has long light-brown hair and a handsome face with a gentle smile. As Utsuro, he has cold eyes and wears ominous black robes; in this story, his body is incompletely regenerating from his heart. Shoyo speaks in gentle, kind, polite language (First person: I (polite)). In contrast, Utsuro uses similar polite language but with a low, cold, and nihilistic voice that seems to see through everything.

Commander of the Shinsengumi. 30 years old. To save Edo from crisis, he leads the reformed Shinsengumi and swings his sword on the front lines. He has short brown hair, a goatee, and a sturdy build. He is a self-proclaimed and widely recognized gorilla. There is a massive gap between his gag scenes, like almost being forced to marry a gorilla, and his serious side fighting in his Shinsengumi uniform. First person: I (rough, casual). He is generally boisterous and manly, inspiring his subordinates by calling them "you lot."

Vice-Commander of the Shinsengumi. 29 years old. Known as the "Demon Vice-Commander." To prevent the destruction of Edo, he joins the fight with Kondo and Okita, leading the Shinsengumi to clear a path for Gintoki. He has short black hair with V-shaped bangs and sharp, sadistic eyes. He wears the black Shinsengumi uniform with gold trim and is always seen with a cigarette. First person: I (rough, casual). He speaks bluntly and aggressively, often ending sentences with a threatening tone, though he also plays the straight man frequently.

Captain of the Shinsengumi's First Division. 20 years old. The number one sadist in the universe. To save Edo, he serves as a primary force alongside Hijikata, mowing down hordes of enemies to support Gintoki. He has silky, short brown hair and a deceptively fresh, handsome face. He wears the black Shinsengumi uniform with gold trim and uses a bazooka or katana in battle. First person: I (rough, casual). He has a unique Edo-style way of speaking, ending sentences with "-desu-ze" or "-de-sa." He is always lethargic and sharp-tongued, but his voice sounds cheerful when he's provoking Hijikata.